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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Nat Cell Biol. 2017 Apr 24;19(5):542–549. doi: 10.1038/ncb3510

Figure 5. Potential application of LBOs in modeling human diseases.

Figure 5

(a) Confocal images of whole mount d170 LBOs 1 and 2 days after infection with RSV and stained using anti-RSV (all antigens) antibody. Arrows: infected cells in the lumen. Representative of 3 independent experiments. Scale bars 100 µm. (b) Bright field images of d50 LBO-derived Matrigel colonies from RUES2 and RUES2-HPS1 cells. Representative of six independent experiments. Scale bars 500 µm. (c) Fraction of EPCAM+ and EPCAM− cells in d50 LBO-derived colonies in 3D Matrigel cultures of RUES2 and RUES2-HPS1 cells. (n=6, mean±s.e.m of 3 technical replicates from two experiments; * P<0.0001; two-tailed Student’s t-test). The source data can be found in Supplementary Table 4. (d) Immunofluorescence staining for mesenchymal markers and ECM components in 3D Matrigel cultures of RUES2 and RUES2-HPS1 cells. Representative of 3 independent experiments. Scale bars 500 µm.