Table 1.

Suggested Flow Cytometry Staining Panels

	Treg panel	T cell ICS	CD8 T cell panel
BV605	CD4 (Biolegend, Cat.#100547)	CD4 (Biolegend, Cat.#100547)	CD4 (Biolegend, Cat.#100547)
APC Cy7	CD29 (Ebioscience, Cat. #47-0291-82)	CD25 (Ebioscience, Cat. #47-0251-82)	CD44 Ebioscience, Cat. #47-0441-82
PerCP eFluor710 (PerCP-Cy5.5)	CXCR3 (Ebioscience, Cat. #45-1831-82)	* IFNg (Ebioscience, Cat. #45-7311-82)	CXCR3 (Ebioscience, Cat. #45-1831-82)
FITC	CD44 (Biolegend, Cat. #103006)	* IL-17 (Biolegend, Cat. #506908)	* Ki67 (Ebioscience, Cat. #11-5698-82)
PE	CCR5 (BD, Cat. #555306)	CD162/PSGL-1 (BD, Cat. #555306)	CCR5 (Biolegend, Cat. #107006)
BUV395		* CD3 (BD, Cat. #740268)	* CD3 (BD, Cat. #740268)
Ax700	* Foxp3 (Ebioscience, Cat. #56-5773-82)	CD62L (Ebioscience, Cat. #56-0621-82)	CD62L (Ebioscience, Cat. #56-0621-82)
PECy7	GITR (Ebioscience, Cat. #25-5874-82)	* Tbet (Ebioscience, Cat. #25-5825-82)	KLRG-1 (Ebioscience, Cat. #25-5893-82)
PECy5	ICOS (Ebioscience, Cat. #15-9942-82)	CD44 (Ebioscience, Cat. #15-0441-83)	CD127 (Ebioscience, Cat. #15-1271-83)
APC	* CTLA-4	* TNFa (Biolegend, Cat. #506308)	NS4b class I tetramer (FHCRC shared resources)
BV421 (Pac Blue)	CD73 (Biolegend, Cat. #127212)	Ly6C (Biolegend, Cat. #128014)	CCR7 (Ebioscience, Cat. #48-1971-82)
BV650	CD25 (Biolegend, Cat. #102037)	CD8 (Biolegend, Cat. #100741)	CD8 (Biolegend, Cat. #100741)
AmCyan	Live/dead (Invitrogen, Cat.#L34957)	Live/dead (Invitrogen, Cat.#L34957)	Live/dead (Invitrogen, Cat.#L34957)

^*denotes intracellular stain