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. 2017 Nov 9;103(1):136–147. doi: 10.3324/haematol.2017.165381

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Figure 2. — DHMEQ treatment activates the intrinsic apoptotic pathway. Densitometrically analyzed protein expression normalized to β-Actin of CLL cells cultured in vitro (A) alone or in co-culture with M2-10B4 cells after 2 days of treatment with 2μg/ml of DHMEQ, (B) alone after 0.5, 1, 2, 4, 8 and 24 hours of treatment with 2 μg/ml of DHMEQ and (C) alone or in co-culture with M2-10B4 cells after 6 days of treatment with 2μg/ml of DHMEQ shown with exemplary western blot. ****P<0.0001, ***P<0.001, **P<0.01 and *P<0.05. D: DHMEQ (dehydroxymethylepoxyquinomicin); D2: day 2; D6: day 6; SD: stroma + DHMEQ; SV: stroma + vehicle control; v: vehicle control; CLL: chronic lymphocytic leukemia; ns: not significant; ACTB: β-Actin.