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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Methods Mol Biol. 2017;1495:57–76. doi: 10.1007/978-1-4939-6451-2_5

Figure 6.

Figure 6

LC–MS analysis of macrocyclic products obtained from in vivo cyclization of Z8C_O2beY precursor protein. (A) LC-MS extracted-ion chromatogram (left panel), MS/MS fragmentation spectrum (middle panel), and chemical structure (right panel) of bicyclic peptide isolated via streptavidin affinity from cells expressing the contruct Z8C_O2beY. (B) Same as (A) for the head-to-tail cyclic peptide byproduct. Peaks labeled with * correspond to unrelated multicharged ions from adventitious proteins.