Skip to main content
. Author manuscript; available in PMC: 2018 Apr 30.
Published in final edited form as: Sci Signal. 2017 Oct 31;10(503):eaan1011. doi: 10.1126/scisignal.aan1011

Figure 7. Mass spectrometry comparison of plasma and serum concentrations of long chain fatty acids C24:4 and C24:5.

Figure 7

The concentration of C24:4 and C24:5 were determined in: (A) plasma from VADC mice treated with or without PHZ; VADC mice, N = 4; VADC + PHZ mice, N = 5; (B) plasma from VAD mice treated with or without PHZ; VAD mice, N = 4; VAD + PHZ mice, N = 4; (C) plasma from NF mice and NFC mice; NFC mice, N = 4; NF mice, N = 5; (D) plasma from NF mice and NFC mice treated with PHZ; NF mice, N = 3; NFC + PHZ, N = 2. (E) serum from mouse and goat analyzed in triplicate; (F) mouse serum extracted by methanol:H2O in 9:1 and 1:1 ratio and analyzed in triplicate. (G) Concentrations of C24:4 and C24:5 extracted from Flag-Gal4-RXRA following immunoprecipitation from 293T-FXP cell extracts treated with mouse serum for 0, 3, 6, 9, 12, or 24 hours, single determination performed at each time point. (H) Concentrations of C24:4 and C24:5 extracted from Flag-Gal4-RXRA following immunoprecipitation of 293T-FXP cells treated with or without mouse serum or goat serum for 12 hrs, analyzed in triplicate (I). Quantification of the fatty acid C24:4 in peripheral blood (PB) and bone marrow (BM) from mice treated with or without PHZ. PBS treated mice, N = 2; PHZ treated mice, N = 2. (J) Quantification of the fatty acid C24:5 in peripheral blood (PB) and bone marrow (BM) from mice treated with or without PHZ by mass spectrometry. PBS treated mice, N = 2; PHZ treated mice, N = 2. Error bars represent standard deviation of individual mice or biological triplicate experiments as indicated. * P < 0.05, ** P < 0.01, *** P < 0.001, T-test.