Table 1.

Enzyme kinetics data

Every data point was repeated three times, and S.E. was calculated by the fitting software. Relative efficiency is defined as enzyme efficiency (k_cat/K_m) divided by that of WT. ND, not determined.

Enzyme	kcat	Km	kcat/Km	Relative efficiency
	s−1	μm	m−1 s−1	%
wDUTWT	7.4 ± 0.2	1.7 ± 0.2	4.4 × 106	100
wDUTR24T	0.45 ± 0.02	2.9 ± 0.3	1.6 × 105	3.6
wDUTD34N	0.92 ± 0.06	13 ± 2	7.1 × 104	1.6
wDUTR71E	ND	ND	ND	ND
wDUTD88N	ND	ND	ND	ND
wDUTS115R	2.6 ± 0.1	4.5 ± 0.5	5.8 × 105	13
wDUTK150A	5.9 ± 0.1	2.5 ± 0.2	2.4 × 106	54
wDUTK150E	5.5 ± 0.1	2.6 ± 0.2	2.1 × 106	49
wDUTR158A	3.2 ± 0.1	2.6 ± 0.2	1.2 × 106	28
wDUTR158E	2.6 ± 0.1	2.6 ± 0.1	1.0 × 106	23
wDUTR158W	3.1 ± 0.1	3.2 ± 0.2	9.7 × 105	22
wDUTV160D	7.4 ± 0.2	2.0 ± 0.2	3.7 × 106	85
wDUTR161K	0.053 ± 0.002	5.4 ± 0.7	9.8 × 103	0.23
wDUTF166Y	1.2 ± 0.1	5.0 ± 0.2	2.4 × 105	5.5
wDUTD170G	6.2 ± 0.2	2.1 ± 0.3	3.0 × 106	68
wDUTD170L	0.53 ± 0.01	7.1 ± 0.4	7.5 × 104	1.7
wDUTR24T/S115R	0.51 ± 0.01	6.7 ± 0.6	7.6 × 104	1.8
wDUTR24T/S115R/D170G	3.1 ± 0.1	4.2 ± 0.4	7.4 × 105	17
wDUTΔ134−153	0.67 ± 0.01	21 ± 1	3.2 × 104	0.73
wDUTΔ161−170	ND	ND	ND	ND
wDUTΔ170−171	1.7 ± 0.1	2.8 ± 0.2	6.1 × 105	14
eDUTWT	9.3 ± 0.2	0.93 ± 0.11	1.0 × 107	100
eDUTR116S	2.7 ± 0.1	5.2 ± 0.7	5.2 × 105	5.2
eDUTT22R/R116S	3.3 ± 0.2	13 ± 2	2.5 × 105	2.5