. 2017 Nov 27;293(3):893–905. doi: 10.1074/jbc.M117.814947

Table 1.

Binding and activation data of ACKR3 mutants

One-way ANOVA with Dunnett's post hoc test is shown in the footnotes (boldface italic type indicates significance). ND, not determined.

Receptor/mutant	Residue number	¹²⁵I-CXCL12 tracer binding (% of ACKR3)	CXCL12				CXCL11
Receptor/mutant	Residue number	¹²⁵I-CXCL12 tracer binding (% of ACKR3)	IC₅₀	Log IC ₅₀ ± S.D.	EC₅₀	Log EC ₅₀ ± S.D.	IC₅₀	Log IC ₅₀ ± S.D.	EC₅₀	Log EC ₅₀ ± S.D.
		%	nm		nm		nm		nm
ACKR3		100	5.9	−8.2 ± 0.1	7.0	−8.2 ± 0.2	39.1	−7.4 ± 0.2	9.8	−8.1 ± 0.2
D90N	2.50	92 ± 13	12.1	−7.9 ± 0.2	247	*−6.7* ± 0.3^a**	35.9	−7.5 ± 0.2	ND	ND
N127D	3.35	70 ± 15^b	10.9	−8.2 ± 0.2	226	*−6.6* ± 0.04^a**	6.6^a	*−8.0* ± 0.2^a**	906^a	*−6.1* ± 0.1^a**
N127K	3.35	83 ± 21	9.9	−8.1 ± 0.2	ND	ND	11.0^a	*−7.9* ± 0.2^b**	ND	ND
N127S	3.35	87 ± 21	1.7	*−8.8* ± 0.2^a**	ND	ND	3.7^a	*−8.5* ± 0.3^a**	ND	ND
D141N	3.49	64 ± 16^a	1.0	*−9.1* ± 0.3^a**	ND	ND	4.8^a	*−8.3* ± 0.2^a**	ND	ND
R142A	3.50	66 ± 12^a	45.1^a	*−7.4* ± 0.2^a**	585^a	*−6.2* ± 0.1^a**	44.8	−7.4 ± 0.2	ND	ND
Y143A	3.51	93 ± 5	3.0	−8.5 ± 0.1	493^b	*−6.4* ± 0.3^a**	15.4^c	−7.8 ± 0.1	436^c	*−6.4* ± 0.3^a**
Y315A	7.53	69 ± 8^b	24.7^b	*−7.7* ± 0.3^a**	ND	ND	19.1^c	−7.7 ± 0.2	ND	ND

^a p < 0.001.

^b p < 0.01.

^c p < 0.05.