Figure 2.

Generation and characterization of CsxA truncation constructs. A, CsxA and CsaB were aligned according to their conserved stealth protein family regions (CR1–CR4). The first and last amino acid, as well as the sites of truncation introduced into the CsxA sequence, are indicated. B, Coomassie-stained SDS-PAGE presenting the purified CsxA constructs. C, activity of the purified CsxA constructs in the absence (−) and presence (+) of primCPSX as determined by use of a spectrophotometric assay. Wild-type activity was set to 100%.