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. 2017 Nov 29;293(3):953–962. doi: 10.1074/jbc.RA117.000488

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Figure 3. — Product profiles of CsxA truncations. A and B, products generated by ΔC98-CsxA (A) and ΔN58-CsxA (B) at increasing d/a ratios after overnight incubation. Oligosaccharide fractions of avDP15 were loaded as marker. The squares indicate the gel positions of primCPSs. C and D, time-course experiments presenting the reaction products catalyzed by ΔC98-CsxA (C) and ΔN58-CsxA (D). The elongation reactions were primed with DP5, and samples were taken at indicated time points. Products were resolved by HPLC-AEC. Previous calibration of the column with individual DPs allowed the assignment of single peaks.