Figure 5.

The double truncated protein ΔN58ΔC99-CsxA is distributive with near Poissonian product profile. A, Coomassie-stained SDS-PAGE showing purified ΔN58ΔC98-CsxA. B and C, SEC experiments carried out with ΔN58ΔC98-CsxA under low-salt (B) and high-salt (C) conditions indicate a mono- to dimeric assembly. D, Alcian blue/silver-stained PAGE comparing the products synthesized by ΔC98-CsxA and ΔN58ΔC98-CsxA. The marker was obtained from the vaccine manufacturer GlaxoSmithKline and is identical to the CPSX fraction of avDP15 used in vaccine production protocols. The dashed line indicates that samples irrelevant for this figure were excised. E, time-course experiment demonstrating reaction products catalyzed by ΔN58ΔC98-CsxA after priming with DP5. The HPLC-AEC profiles indicate a near Poissonian distribution of products over the entire time frame.