. 2017 Nov 29;293(3):953–962. doi: 10.1074/jbc.RA117.000488

Table 1.

Primers used in this study

The restriction sites are highlighted in bold type.

Primer pair	Resulting construct
`CGGGATCCCCAATTGAAGATCCATACCCAGTA`	pMBP-ΔN58-csxA-His₆
`CCGCTCGAGTTGTCCACTAGGCTGTGATG`
`GCGGATCCATTATGAGCAAAATTAGCAAATTG`	pMBP-ΔC98-csxA-His₆^a
`CCGCTCGAGGAGAATTTCTGCTTCTGATACATC`
`CGGGATCCCCAATTGAAGATCCATACCCAGTA`	pMBP-ΔN58ΔC98-csxA-His₆
`CCGCTCGAGGAGAATTTCTGCTTCTGATACATC`
`GCATCTCATATGCTTCCTACTAAATCTGAAGTAGCTG`	pΔN387-csxA^a
`GCATCTCTCGAGTTGTCCACTAGGCTGTGATGTG`
`GCATCTCATATGCTGATCCCGATCAATTTCTTT`	pΔN69-csaB_co-csaA-His6
`GCGGATCCGTTATTGTTATTGTTGTTGTTATTGTTATTGGAGCTCGATTTCTCGAAGGAGCTCGGCAACGG`
`GCGGATCCAAAGTCTTAACCGTCTTTGGC`	pΔN69-csaB_co-*csaA*-His6
`CCGCTCGAGTCTATTCTTTAATAAAGTTTCTACA`

^a Leucine 388 of the CsxA sequence, normally encoded by ctt, is encoded by ctc from the XhoI site used for cloning.