Figure 2.

Macroscopic Cl_Ca currents in rat pinealocytes. A, in whole-cell voltage-clamp experiments, single pinealocytes were depolarized from the holding potential of −40 mV to test potentials (−80 ∼ +100 mV) by +20 mV increment for 500 ms and subsequently repolarized to −80 mV for 250 ms every 15 s. Representative current traces of pCa 7.0, 6.5, and 6.0 in the pipette solution. Note that time-dependent outward currents and tail currents, which are characteristic of Cl_Ca currents, were observed at pCa 6.5 and 6.0 in pinealocytes. B, current-voltage relationships of outward currents at pCa 7.0, 6.5, and 6.0. Note that these currents reversed around 0 mV, which is a theoretical equilibrium potential of Cl⁻ under the experimental conditions. C, Ca²⁺ dependence of outward currents. D, τ_act of outward currents. E, τ_tail of tail currents. Experimental data were obtained from 5 to 10 pinealocytes.