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. 2018 Jan 17;11:133. doi: 10.3389/fnana.2017.00133

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Copyright © 2018 Albert-Gascó, Ma, Ros-Bernal, Sánchez-Pérez, Gundlach and Olucha-Bordonau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of neurons expressing Rxfp3, vGAT (slc32a1), and ChAT mRNA, relative to DAPI-stained nuclei in the rat MS, SFi, and LSI at bregma +0.24 mm (A) and a schematic map illustrating different neuronal phenotypes based on mRNA co-expression, and their distribution (B). Dotted lines indicate the midline and the medial and lateral septal and septofimbrial borders. High-magnification images illustrate co-localization of Rxfp3 (C,G,K), ChAT (D,H,L), vGAT (E,I,M) mRNA and merged signals (F,J,N) in the MS, SFi, and LSI, respectively. No co-localization of Rxfp3 and ChAT mRNA was observed (open arrowheads). Calibration bar in (A) 125 μm and (C–N) 50 μm.