REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-EZH2	Cell Signaling Technology	Cat #5246
Rabbit monoclonal anti-SUZ12	Cell Signaling Technology	Cat #3737
Rabbit monoclonal anti-AMPKα	Cell Signaling Technology	Cat #5831
Rabbit monoclonal anti-Phospho-AMPK Substrate Motif LXRXX(pS/pT)	Cell Signaling Technology	Cat #5759
Rabbit monoclonal anti-Phospho-T172-AMPKα	Cell Signaling Technology	Cat #2535
Rabbit monoclonal anti-ACC	Cell Signaling Technology	Cat #3676
Rabbit monoclonal anti-Phospho-S79-ACC	Cell Signaling Technology	Cat #11818
Rabbit monoclonal anti-RBBP4	Cell Signaling Technology	Cat #9067
Rabbit monoclonal anti-histone H3	Cell Signaling Technology	Cat #4499
Rabbit monoclonal anti-H3K27me2	Cell Signaling Technology	Cat #9728
Rabbit monoclonal anti-H3K27me3	Cell Signaling Technology	Cat #9733
Rabbit monoclonal anti-H3K4me3	Cell Signaling Technology	Cat #9727
Rabbit monoclonal anti-H3K9me2	Cell Signaling Technology	Cat #4658
Rabbit monoclonal anti-H3K36me2	Cell Signaling Technology	Cat #2901
Rabbit monoclonal anti-H3K79me2	Cell Signaling Technology	Cat #5427
Rabbit monoclonal anti-Phospho-S10-H3	Cell Signaling Technology	Cat #3377
Rabbit polyclonal anti-Phospho-T311-EZH2	Cell Signaling Technology	Cat #27888
Rabbit polyclonal anti-EED	Abcam	Cat #ab4469
Mouse monoclonal anti-c-Myc	Santa Cruz	Cat #sc-40
Rabbit polyclonal anti-HA	Santa Cruz	Cat #sc-805
Mouse monoclonal anti-HA	Santa Cruz	Cat #sc-7392
Mouse monoclonal anti-HDAC5	Santa Cruz	Cat #sc-133106
Mouse monoclonal anti-TUBULIN	Sigma-Aldrich	Cat #T5168
Mouse monoclonal anti-VINCULIN	Sigma-Aldrich	Cat #V4505
Mouse monoclonal anti-Flag	Sigma-Aldrich	Cat #F3165
Rabbit polyclonal anti-Flag	Sigma-Aldrich	Cat #F2425
Mouse monoclonal anti-Flag agarose beads	Sigma-Aldrich	Cat #A2220
Mouse monoclonal anti-HA agarose beads	Sigma-Aldrich	Cat #A2095
peroxidase-conjugated anti-mouse secondary antibody	Sigma-Aldrich	Cat #A4416
peroxidase-conjugated anti-rabbit secondary antibody	Sigma-Aldrich	Cat #A4914
IKKα	Cell Signaling Technology	Cat #2682
Rabbit polyclonal anti-acetylated H3	EMD Millipore	Cat #06-599
Bacterial and Virus Strains
XL10 Gold Escherichia coli	Agilent	Cat #200314
BL21(DE3) Escherichia coli	Dr. William G. Kaelin, Jr., Dana-Farber Cancer Institute	N/A
Biological Samples
Ovarian tumor samples (n=235)	University of Texas MD Anderson Cancer Center	N/A
Breast tumor samples (n=210)	University of Texas MD Anderson Cancer Center or Mackay Memorial Hospital (Taipei, Taiwan)	N/A
Chemicals, Peptides, and Recombinant Proteins
A-769662	Selleck Chemicals	Cat # S2697; CAS: 844499-71-4
Dorsomorphin (Compound C) 2HCl	Selleck Chemicals	Cat # S7306; CAS: 1219168-18-9
Metformin HCl	Selleck Chemicals	Cat # S1950; CAS: 1115-70-4
2-Deoxy-D-glucose	Sigma-Aldrich	Cat # D8375; CAS: 154-17-6
Cycloheximide	Sigma-Aldrich	Cat # C7698; CAS: 66-81-9
Chicken polynucleosomes, purified	Epicypher	Cat # 16-0004
AMPK (A1/B1/G2) Kinase	Promega	Cat # V4012
Critical Commercial Assays
ThruPLEX-FD Prep Kit	Rubicon Genomics	R400427
True-seq RNA sample preparation kit	Illumina	RS-122-2001
Deposited Data
Raw and analyzed sequencing data	This paper	GEO: GSE97736
Raw data and images	This paper and Mendeley Data	http://dx.doi.org/10.17632/jdf8ddt32n.1
Experimental Models: Cell Lines
Human: 293T cells	Dr. William G. Kaelin, Jr., Dana-Farber Cancer Institute	N/A
Human: T98G cells	Dr. William G. Kaelin, Jr., Dana-Farber Cancer Institute	N/A
Human: OVCAR5 cells	Dr. Marc W. Kirschner, Harvard Medical School	N/A
Human: OVCAR8 cells	Dr. Marc W. Kirschner, Harvard Medical School	N/A
Human: PC3 cells	Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center	N/A
Human: MDA-MB-231 cells	ATCC	CRM-HTB-26
Human: ZR-75-1 cells	ATCC	CRL-1500
Human: MDA-MB-435 cells	ATCC	HTB-129
Human: MCF7 cells	ATCC	HTB-22
Mouse: WT and Ampkα1(−/−);Ampkα2(−/−) Mouse Embryonic Fibroblasts (MEFs)	Dr. Benoit Viollet, Institut Cochin, Paris, France	N/A
Experimental Models: Organisms/Strains
Mouse: NCRNU-F: CrTac:NCr-Foxn1nu	Taconic Biosciences	NCRNU-F
Oligonucleotides
Primers for real-time RT-qPCR, see Table S1	This paper	N/A
Recombinant DNA
pCMV-HA-EZH2	Addgene	Cat # 24230
pGEX-EZH2	Addgene	Cat # 28060
pET28a-EZH2	This paper	N/A
Mouse EZH1 cDNA	Dr. Stuart H. Orkin, Dana-Farber Cancer Institute	N/A
pEBG-AMPKα1(1-312)	Addgene	Cat # 27632
pDONR223-PRKAA1	Addgene	Cat # 23871
pDONR223-PRKAA2	Addgene	Cat # 23671
pCMVHA-EED	Addgene	Cat # 24231
pCMVHA-SUZ12	Addgene	Cat # 24232
pCMV-Flag-EZH2	This paper	N/A
pLenti-CMV-hygro-HA-EZH2	This paper	N/A
Myc-PLU1	(Klose et al., 2007)	N/A
Myc-PCL1	(Klose et al., 2007)	N/A
HA-AEBP2	(Klose et al., 2007)	N/A
HA-UTX	(Klose et al., 2007)	N/A
HA-JMJD3	(Klose et al., 2007)	N/A
pLKO-shEZH2	(Xu et al., 2012)	N/A
Software and Algorithms
ImageJ	Freeware	N/A
Prism V7	Graphpad	N/A
Other