Figure 2. Requirements for three Irs for rejecting Ca²⁺-containing food.

(A) Screening candidate chemoreceptors for defects in Ca²⁺ aversion. n=4—12.

(B) Dose-dependent avoidance of Ir mutants to Ca²⁺. n=4.

(C) Rescue of Ca²⁺ avoidance defects in Ir25a² by expressing UAS-Ir25a using the indicated GAL4, or the Ir25a⁺ genomic transgene (gen. Ir25a). n=5—12.

(D) Rescue of Ca²⁺ avoidance deficits in Ir62a¹ by expressing UAS-Ir62a using the indicated GAL4. n=4.

(E) Rescue of Ca²⁺ avoidance impairments in Ir76b¹ by expressing UAS-Ir76b using the indicated GAL4. n=5—13. Error bars represent SEMs. Asterisks in A and B indicate significant differences from controls (*P < 0.05, **P<0.01) using ANOVA with Scheffe’s post hoc test. The black and red asterisks in C—E indicate significant differences from the controls and mutants, respectively (*P < 0.05, **P<0.01), using ANOVA with Scheffe’s post hoc test.