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. Author manuscript; available in PMC: 2019 Jan 3.
Published in final edited form as: Neuron. 2017 Dec 21;97(1):67–74.e4. doi: 10.1016/j.neuron.2017.11.038

Figure 4. Labellar expression of IR25a, and toxicity of high Ca2+.

Figure 4

(A—O) Staining of labella from controls viewed by confocal microscopy. 3-D reconstructions generated by maximum transparency. Scale bars represent 25 μm.

(A, D, G, J, M) UAS-GFP expression driven by the indicated GAL4s. The signals were detected by anti-GFP staining (green).

(B, E, H, K, N) Anti-IR25a staining (red).

(C, F, I, L, O) Merged images of anti-GFP and anti-IR25 staining.

(P and Q) Survival of control flies fed 100 mM fructose or 100 mM fructose mixed with: (P) CaCl2, or (Q) MgCl2. n=4—7. Error bars represent SEMs. The asterisks indicate significant differences from the fructose only feeding (**P<0.01, *P<0.05.) using single factor ANOVA with Scheffe’s as a post hoc test to compare two sets of data.