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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cancer Lett. 2017 Dec 13;416:11–23. doi: 10.1016/j.canlet.2017.12.008

Fig. 1. SEC inhibited the cell migration of HEK 293T RKIP−/− cells.

Fig. 1

(A) RKIP protein level in HEK293T RKIP+/+ and RKIP−/− cells. (B) A scratch on HEK293T RKIP+/+ and RKIP−/− cells was made, followed by incubation with SEC (20 µM) for 24 h. Relative wound closure was quantified by measuring the width of the wounds. (C) A scratch was made on HEK293T RKIP−/− cells transfected with pCMV6 empty vector and pCMV6-RKIP plasmid for 24 h, then treated with 20 µM SEC for 24 h. The width of the wounds was measured and relative wound closure was quantified. (D) HEK293T RKIP+/+ and RKIP−/− cells were treated with 20 µM SEC for 6, 12 and 24 h. The protein level of epithelial marker E-Cadherin and mesenchymal marker Vimentin was examined by western blot. Data are mean ± SEM; * p < 0.05, ** p < 0.01, NS p > 0.05, n = 3.