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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cancer Lett. 2017 Dec 13;416:11–23. doi: 10.1016/j.canlet.2017.12.008

Fig. 2. SEC inhibited PC3 cell migration.

Fig. 2

(A) PC3 cells were treated with 20 µM SEC for 24 h after making a scratch. Then the width of the wounds was measured and relative wound closure was quantified. (B) Overexpression of RKIP in PC3 cells inhibited cell migration. A scratch was made on PC3 cells transfected with pCMV6 empty vector or pCMV6-RKIP plasmid for 24 h. The width of the wounds was measured and relative wound closure was quantified. (C) PC3 and LNCaP cells were incubated with 20 µM SEC for 6, 12 and 24 h. Western blot analysis of the protein level of epithelial marker E-Cadherin and mesenchymal marker Vimentin. Data are mean ± SEM; * p < 0.05, ** p < 0.01, n = 3.