Figure 2.

Genipin induces G0/G1 cell cycle arrest in bladder cancer cells. (A) Flow cytometry analysis of propidium iodide-stained cells following genipin treatment for 48 h. Representative histograms of cell cycle distribution profiles are presented in the top panels. Bottom panels demonstrate quantification of the percentage of cells in each cell cycle phase. (B) Western blot analysis of indicated proteins in cells with or without genipin (30 or 60 µM). Bar graphs demonstrate densitometric analysis of western blots from three independent experiments. *P<0.05 vs. vehicle-treated cells. CDK, cyclin-dependent kinase; p21, cyclin-dependent kinase inhibitor 1; p27, rabbit anti-cyclin-dependent kinase inhibitor 1B.