Table I.

Characterization of MTX-loaded liposomes.

Formulation	PC:CL: PEG 400	MTX: lipid	Particle size, nm	PDI	Zeta potential, mV	Entrapment efficiency, %
M-LF	55:40:5	1:20	105.7±5.5	0.13	8.1±3.7	54.6±3.5
M-LN	60:35:5	1:20	111.8±2.8	0.34	22.4±1.2	67.2±1.5
M-LP	60:35:5	1:10	137.4±2.6	0.31	36.0±3.1	73.4±1.7

Data are presented as the mean ± standard deviation (n=3). F, N and P denote the identity of the MTX-entrapped liposomes with distinct compositions. MTX, methotrexate; PC, phosphatidylcholine; CL, cholesterol; PEG, poly(ethylene glycol); PDI, polydispersity index.