Figure 3. Migration of stem cells from human exfoliated deciduous teeth (SHED) toward medium conditioned by incubation with calcium hydroxide (CH), mineral trioxide aggregate (MTA) or BiodentineTM (BD). Serum-starved SHED could migrate through 8-μm pore-size inserts (in companion plates) overnight, and the number of migrating cells was estimated by staining with Cell TrackerTM Green CMFDA. Regular growth medium (alpha-MEM with 10% FBS) or medium with 20% FBS were used as negative and positive control, respectively. Data represent mean ± SD (n=3 independent experiments, performed in triplicates). *p<0.05 (one-way analysis of variance).
