Fig. 5.

Reflection of molecular changes associated with TMZ resistance in GSC-derived EVs. (A) TMZ effects on cell viability (MTS assay). (B, C) Nanoparticle tracking analysis of EVs produced by SGSC lines in the absence (B) or presence of 100 μM TMZ (C). (D, E) Expression of transcripts implicated in TMZ resistance in GSCs and in their EVs. (D) Quantitative PCR analysis reveals changes in EV-associated TGM2, MGMT, and/or Nestin (1 = average mRNA expression in TMZ-sensitive cell lines). (E) The comparison of heatmaps between cell and EV profiles shows a great similarity. *P < 0.05 (N = 2).