Fig. 3.

GMCI induces an increase in PD-L1 levels in vivo. (A) Immunofluorescent staining of PD-L1 in untreated and GMCI treated GL261Luc2-bearing mice. Nuclei were stained with Hoechst (blue), tumor cells stained for vimentin (red) and PD-L1 (green). Scale bars: 20 µm. (B) Flow cytometry analysis of PD-L1 expression in infiltrating macrophages and microglia in the brains of mice bearing GL261Luc2 tumors at day 21. Macrophages were gated CD45^highCd11c-Cd11b+ and microglia were gated CD45^lowCd11c-C11b+. Long horizontal bars indicate the mean values. Shorter horizontal bars indicate the standard deviation. One-way ANOVA was used to determine statistical significance (***P ≤ 0.001, **P ≤ 0.01).