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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Dev Neurobiol. 2017 Jul 25;77(10):1221–1236. doi: 10.1002/dneu.22512

Table 2.

Impact of Neonatal Infection on Circulating Peripheral WBCs, Including Lymphocytes, Neutrophils, Monocytes, Eosinophils, and Basophils in Juvenile Male and Female Rats Following a Mild Immune Challenge of LPS (25 μg/kg)

Cell Type Male
Female
Statistics
Saline
E. coli
Saline
E. coli
Saline LPS Saline LPS Saline LPS Saline LPS
Total WBC (k/μL)   2.97 ±0.21     3.2 ±0.35   2.45 ± 0.37   1.72 ±0.22†   2.61 ± 0.24   3.41 ± 0.48   3.18 ±0.46   2.41 ± 0.45† Neonatal Trmt × P24 Trmt:
F1,72 = 6.081;
P = 0.016
Lymphocytes (k/μL)   1.62 ±0.13   1.46 ± 0.20#   1.42 ±0.27   0.92 ±0.14†   1.48 ±0.17   1.43 ±0.18#   1.3 ±0.09   1.06±0.28† P24 Trmt:
F1,72 = 4.117;
P = 0.046
Neutrophils (k/μL)   1.16 ±0.12   1.61 ± 0.24   0.84 ± 0.13†   0.75 ± 0.08†   0.97 ±0.10   1.8 ±0.29   1.41 ± 0.23   1.26 ±0.20 Sex × Neonatal Trmt:
F1,72 = 6.201;
P = 0.015
Neonatal Trmt × P24 Trmt:
F1,72 = 5.599;
P = 0.021
Monocytes (k/μL)   0.16 ±0.04   0.12 ± 0.03#   0.10 ± 0.02*   0.06 ± 0.01†   0.15 ± 0.03   0.15 ± 0.04#   0.15 ± 0.04*   0.07 ± 0.02*# Neonatal Trmt:
F1,72 = 5.882;
p = 0.018
P24 Trmt:
F1,72 = 4.810;
p = 0.032
Eosinophils (k/μL) 0.008 ± 0.002 0.013 ± 0.0021 0.013 ± 0.004 0.013 ±0.004 0.007 ± 0.003 0.023 ± 0.005 0.019 ± 0.008 0.015 ± 0.004 NS
Basophils (k/μL) 0.001 ± 0.001 0.001 ± 0.001 0.025 ± 0.024 0.001 ± 0.001 0.001 ± 0.001 0.006 ± 0.003 0.016 ±0.010 0.002 ± 0.001 NS
% Lymphocytes 55.36 ± 2.93 45.71 ±3.57 58.59 ± 4.06 50.67 ± 2.4 56.37 ± 3.24 43.44 ± 1.95 50.48 ± 3.99 41.11 ± 3.81
% Neutrophils 38.56 ± 2.17 50.36 ± 3.37 32.52 ± 2.05 45.09 ± 2.3 37.63 ± 3.01 51.7 ± 1.76 43.93 ±4.08 55.33 ± 3.93
% Monocytes   5.73 ± 1.11   3.31 ± 0.59   4.16 ± 0.72   3.13 ±0.24   4.85 ± 0.66   3.84 ± 0.68   4.27 ± 0.56   2.79 ± 0.47
% Eosinophils 0.295 ± 0.073 0.429 ± 0.085 0.461 ± 0.115 0.637 ±0.128 0.304 ± 0.087 0.740 ±0.183 0.656 ± 0.235 0.673 ± 0.160
% Basophils 0.034 ± 0.012 0.077 ± 0.032 0.078 ± 0.030 0.115 ± 0.030 0.048 ± 0.031 0.280 ± 0.115 0.207 ± 0.102 0.084 ± 0.024

Data are presented as the mean ± SEM for each cell type.

*

P < 0.05 indicates a main effect of Neonatal Treatment;

#

P < 0.05 indicates a main effect of P24 Treatment;

†

P < 0.05 indicates significant post hoc tests. The percentage of WBCs that individual cell types represent is listed for reference.