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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Clin Cancer Res. 2017 Jul 5;23(19):5892–5911. doi: 10.1158/1078-0432.CCR-17-0001

Figure 5.

Figure 5

Figure 5

Figure 5

Figure 5

Figure 5

Figure 5

Figure 5

Reduced tumor growths and metastasis in vivo after shRNAenv downregulation of HERV-K. A-C, significantly slower growth was demonstrated in three PC cell lines transfected with shRNAenv compared with shRNAc. Smaller tumor sizes (top panels), reduced grow curves (bottom left panel), and reduced tumor weights (bottom right panels) were observed when Panc-1 (A: P = 0.0074 for nude mice and P = 0.0103 for NSG mice), Panc-2 (B: P = 0.0205 for nude mice and P = 0.0003 for NSG mice), or BxPC-3 (C: P = 0.0021 for NOD/SCID mice) cells transduced with shRNAenv were compared with those transduced with shRNAc cells. D, IHC was used to determine the expression of HERV-K or RAS in BxPC-3 tumors. Reduced expression of HERV-K and RAS was demonstrated in tumors expressing shRNAenv. Increased expression of TP53 was detected in tumors expression shRNAenv. E. GFP-positive Panc-2 cells were observed in three lung biopsies of mice bearing xenografts of Panc-2 cells transduced with shRNAc) but not with shRNAenv. Pictures were taken from the bottom of the dishes where the lung biopsies had been cultured for 3 weeks. For statistical analysis, an unpaired two-tailed t-test was used.