Figure 2. Targeted disruption of the p53RE in PURPL uncovers a pro-survival function of PURPL.
(A) (Top) Schematic showing the p53RE in the PURPL promoter and location of the guide RNAs. (Bottom) RT-qPCR analysis from HCT116 PURPL-WT cells (WT#1 and WT#2) and HCT116 PURPL-KO cells (KO#1 and KO#2) untreated or treated with DOXO for 16 hr. (B) PURPL-WT and PURPL-KO cells were untreated or treated with DOXO for 48 hr; cell death (sub-G1 cells) and effect on cell cycle was assessed by PI staining followed by FACS analysis. (C) Immunostaining for Nucleoporin and cleaved caspase-3 from PURPL-WT and PURPL-KO clones with or without DOXO treatment (72 hr). DNA was counterstained with DAPI. Error bars represent SD from 3 experiments. **p<0.01 and ***p<0.001.