Figure 1.

MRP1 and BCRP mRNA and protein expression is altered between M1 and M2 U937 macrophages. U937 cells were polarized to the M1 or M2 phenotype, or left unstimulated for 48 h. After 48 h, cells were collected, and mRNA was isolated for real-time PCR, while cell lysates were collected for Western blotting. Real-time PCR and Western blotting were performed for MRP1 and BCRP. (a) and (b) show real-time PCR results for three replicates of the experiments, with results from each replicate normalized to the unstimulated condition. (c) and (d) show representative Western blots from three replicates, while (e) and (f) show average densitometry normalized to the unstimulated condition in each blot. Groups were compared via one-way ANOVA with Tukey’s post-test, and *p value < 0.05 vs. M1 cells, ^p value < 0.05 vs. M2 cells.