Figure 4.

Inhibition of MRP1 function decreases viral replication in lopinavir-treated U1 cells in a phenotype-specific manner. U1 cells were polarized to the M1 or M2 phenotype with cytokines for 48 h. After polarization, cells were treated with either 10 or 27 LPV (a, b), the MRP1 inhibitor MK571, or a combination of the two. Supernatants were collected, and p24 production was analyzed via ELISA. P24 production was normalized by the untreated condition for each phenotype. Groups were compared via two-way ANOVA with Tukey’s post-test, and significance calculated with a p value < 0.05 vs. *untreated cells, ^LPV-treated cells, # MK571-treated cells.