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Impact of TSA on HDAC activity and histone acetylation. A, TSA diminishes HDAC activity after 8 hours. Columns, means of triplicate values of two independent experiments; bars, SD. B, TSA increases histone acetylation after 12 hours. Proteins were quantitated and fractionated by SDS-PAGE, transferred to nitrocellulose membranes, and probed with rabbit antibodies against total H4 (11 kDa) and H2B (14 kDa) and acetylated histones AcH4 (11 kDa) and AcH2B (14 kDa).
