Figure 1. Schematic showing experimental plan for lentiviral transduction of blastocysts and downstream analyses.

Blastocysts are transduced with lentiviral particles (shown in green) expressing specific ‘gain-of-function’ or ‘loss-of-function’ constructs and cultured for 72 h for outgrowth assays or directly transferred to day 3.5 pseudopregnant animals for in vivo analyses.