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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Cond Med. 2017 Dec;1(1):2–8.

Figure 1. Schematic of modified segregated astrocyte-neuronal co-culture system.

Figure 1

Confluent astrocytes were plated in a cell culture dish containing paraffin pedestals. Following exposure to IPC or Sham preconditioning, neurons (10-14 DIV) were placed on top of the pedestals. The paraffin pedestals allowed for physical separation of neurons and astrocytes but free exchange of soluble mediates. Neurons were incubated with IPC- or Sham-preconditioned astrocytes for 48 hours. After 48 hours, coverslips were removed and placed in a new cell culture dish, and were exposed to lethal OGD (3 hr). 48 hours following termination of lethal OGD, cell death was assayed in neuronal cultures with LDH release assay.