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Selectivity of UE4J sensor. Sensor response after (a) immobilization of USL probe and backfilling with MCH (b) incubation with m-MeB, f1 and miRNA-122 (5′-UGG-AGU-GUG-ACA-AUG-GUG-UUU-G-3′; 50 nM) (c) addition of a single base mismatch (50 nM) and (d) a four times excess of single base mismatch (200 nM).
