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(A) Successive steps for the preparation of matrix-bound SDF-1α using layer-by-layer films as ECM matrix with poly(L-lysine) (PLL) and hyaluronan (HA) as polyelectrolytes. The film is first deposited step-by-step (1), then cross-linked (2) and finally loaded with SDF-1α in acidic conditions (1 mM HCl) (3), followed by a rinsing step in order to obtain matrix-bound SDF-1α (4). (B) SDF-1α can be presented as a soluble cue (sSDF) or in a matrix-bound manner (bSDF).
