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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Biomaterials. 2017 Feb 27;127:61–74. doi: 10.1016/j.biomaterials.2017.02.035

Figure 5.

Figure 5

Effects of CXCR4, CD44 and β1 integrin silencing on bSDF-induced cell spreading and protrusion formation. CD44, CXCR4 and β1 integrin were knocked-down in MDA-MA-232 cells using specific siRNAs before cells were seeded onto bSDF films for 16 h. (A) Representative images of cancer cells after receptor silencing of CXCR4, CD44 and β1 integrin in comparison to control siRNA; (B) Quantitative analysis of the cell spreading area, circularity and (C) aspect ratio. (D) The quantitative analysis of the distribution of the four protrusion phenotypes is also given. For the quantifications, at least 50 cells were analyzed for each experimental condition in each independent experiment. * p < 0.05 in comparison to control (Anova one way on ranks). Three independent experiments were performed. (E) Western Blot showing the effective decrease of receptor expression (CXCR4, CD44, β1 integrin) after siRNA-mediated silencing. Cells were transfected with a scrambled siRNA as a control.