Table 1. Cryo-EM data collection, refinement and validation statistics.
| hINO80 core complex (EMD-3772) | High resolution hINO80 sub-complex (EMD-3773 PDB 5OAF) | SC2 (EMD-3774) | SC2plus (EMD-3775) | |
|---|---|---|---|---|
| Data collection and processing | ||||
| Magnification | 123,000 | 123,000 | 62,000 | 62,000 |
| Voltage (kV) | 300 | 300 | 200 | 200 |
| Electron exposure (e–/Å2) | 45-50 | 45 | 43 | 50 |
| Defocus range (μm) | -1.0 to -3.5 | -1.2 to -3.2 | -1.3 to -4.5 | -1.0 to -4.0 |
| Pixel size (Å) | 2.26 | 1.06 | 3.30 | 3.30 |
| Symmetry imposed | none | none | none | none |
| Initial particle images (no.) | 367,000 | 304,304 | 231,000 | 96,533 |
| Final particle images (no.) | 11,496 | 104,214 | 74,226 | 85,989 |
| Map resolution (Å) | 9.6 | 4.1 | 11.5 | 8.4 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | ~7 to ~15 | ~3 to ~8 | ~10 to ~15 | ~8 to ~11 |
| Refinement | ||||
| Initial model used | 4WVY | |||
| Model resolution (Å) | 4.1 | |||
| FSC threshold | 0.143 | |||
| Model resolution range (Å) | 4-8 | |||
| Map sharpening B factor (Å2) | -140 | |||
| Model composition | ||||
| Nonhydrogen atoms | 19748 | |||
| Protein residues | 2,538 | |||
| Ligands | 6 | |||
| B factors (Å2) | ||||
| Protein | 75 | |||
| Ligand | 28 | |||
| R.m.s. deviations | ||||
| Bond lengths (Å) | 0.23 | |||
| Bond angles (°) | 0.41 | |||
| Validation | ||||
| MolProbity score | ||||
| Clashscore | 12.2 | |||
| Poor rotamers (%) | 0.00 | |||
| Ramachandran plot | ||||
| Favored (%) | 97.6 | |||
| Allowed (%) | 2.4 | |||
| Disallowed (%) | 0 | |||