Skip to main content
. 2017 Dec 6;8(70):114474–114480. doi: 10.18632/oncotarget.22967

Figure 2.

Figure 2

(A) MyLa cells were treated with volasertib and BRD4 binding to the c-myc locus examined by Chromatin immunoprecipitation assay (ChIP). (B) MyLa cells were treated with volasertib and c-myc gene expression was examined by quantitative real-time PCR. (C) C-myc phosphorylation following volasertib (Vola) treatment (overnight) in MyLa cells was examined by immunoblotting. (D) MyLa cells (2 × 106) were injected subcutaneously in immunodeficient NSG mice. Upon tumor engraftment, volasertib (30 mg/kg) or vehicle control (DMSO) were injected intraperitoneally in 50 μL total volume (n = 10/group). Tumors were measured and mice humanely euthanized 4 days later. (E) Expression of c-myc examined by immunohistochemistry from representative samples of tumor xenografts with or without volasertib treatment.