Figure 5. Effects of 5-FU, DAC, and nanoparticles on MKN28 cell viability.
Cell viability was assessed by the MTT every 24h for 3 days. Saline was used as treatment control (A). Control NPs and TNPs (no drug), and free DAC mildly reduced cell viability. Sequential administration of free DAC and 5-FU showed a similar suppression effects with 5-FU alone (P > 0.05). Sequential treatment with DAC encapsulated in different nanoparticles and 5-FU exhibited similar suppressive effects compared with both 5-FU and DAC 72h + 5-FU (P > 0.05) after the treatment of 24h (B), 48h (C) and 72h (D). DAC, 5-Aza-2′-deoxycytidine; NPs, nanoparticles; TNPs, gelatinases-stimuli nanoparticles; 5-FU, 5-fluorouracil.