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. 2017 Oct 3;8(70):114540–114553. doi: 10.18632/oncotarget.21457

Figure 1. SIRT1 shows increased expression and associates with DNMT3B during stress condition.

Figure 1

The expression of γH2AX (A), SIRT1 (B) and H4K16ac (C) were evaluated by western blot in adhered (ma) and deadhered (D24h) melan-a melanocytes. The expression of β-ACTIN or ERK was used as endogenous control. The interaction between SIRT1 and DNMT3B (D) was determined by co-immunoprecipitation in adherent (ma) and deadhered (D24h) melanocytes. Total: Total protein extract used in IP assay; IP: immunoprecipitated protein fraction.