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. 2017 Dec 9;8(70):114576–114587. doi: 10.18632/oncotarget.23051

Figure 4. Expression of PD-L1 and YAP after YAP forced over-expression in siRNA-YAP H460 cells.

Figure 4

(A–B) qRT-PCR analysis of mRNA levels of YAP and PD-L1 after YAP silencing by siRNA and/or forced over-expression of the YAP gene in H460 cells. (C) Western blot analysis of YAP, PD-L1 after YAP silencing by siRNA and/or forced over-expression of the YAP gene in H460 cells. GAPDH was detected as a loading control. Band intensity was analyzed with ImageJ software and normalized with the intensity of GAPDH band. *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001.