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. 2017 Sep 27;8(70):114677–114684. doi: 10.18632/oncotarget.21309

Figure 3. PPP1R1C is a bona-fide target of miR-182 in the glioblastoma cell line U87-MG.

Figure 3

(A) Steady state expression of miR-182 and PPP1R1C in U87-MG and HCN-2 cell lines were determined. Data was normalized to RNU6B and TBP expression, respectively. Fold expression in U87-MG cells was determined relative to expression in HCN-2 cell line. (B) Relative luciferase activity of transiently transfected luciferase reporter constructs containing either full-length or mutated (miR-182 binding site deleted) PPP1R1C 3′ UTR in U87-MG and HCN-2 cells, either mock transfected or transfected with miR-182 mimic or miR-182 antagomir. *p<0.05. Data in ‘A’ and ‘B’ represent at least three independent experiments, each done in triplicate.