Figure 1. Expression profile of HSPA1A/HSPA5, HSPA5, HSPA8, XBP-1 and Beclin-1 genes by real-time quantitative PCR in RPMI8226 (WT) versus RPMI8226-LUC-PURO cell lines.

(A) and (B) U266 (WT) versus U266-LUC-PURO cell lines. Relative expression was performed using 2-^ΔΔCt formula and β-actin housekeeping gene. The analyses were done in triplicates. X axis: Genes; Y axis: fold change value. There was no statistically significant difference in the expression of all genes when comparing the two cell lines, with or without transduction with luciferase gene (p > 0.05). One-Way ANOVA, with Bonferroni’s post -test.