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. 2017 Oct 10;8(70):114756–114768. doi: 10.18632/oncotarget.21801

Figure 1. Cellular viability assessed by the mitochondrial function, differential expression of Ras, p44/42-mapk, GSK3ß and MMP-9.

Figure 1

(a) Schematic depiction of the experimental design employed for this study; (b) a dose-response curve to drug treatment - Cytotoxicity of doxorubicin at various concentrations for MCF7 or MCF7Res cells was determined following 24h drug treatment as described in Methods. The means of at least three independent experiments are plotted. Cell viability was assessed by the ability of cells to reduce MTT salt (vital stain) as described in Materials & Methods. Our results show a difference in the viability of the tested lineages. Cells were cultured under routine classic conditions. In the semi-confluence, the cells were lysed using standard lysing buffer (described in M&M) and resolved on SDS-PAGE gel following transfer to PVDF membranes and staining using specific primary antibodies; (c) representative blots following probing for Ras, p44/42-mapk (Erk 1/2), GSK3ß and MMP-9 are shown, as well as their arbitrary values obtained by densitometric analysis; (d-g, respectively) normalized to the average values of the respective bands of ß-Actin for the appropriate conditions. # means statistical significance (test t-student). The ß-Actin was used as loading sample (approximately 75μg of protein per lane). The differences were significant when *p<0.05.