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. 2017 Oct 19;8(70):114787–114800. doi: 10.18632/oncotarget.21918

Table 2. Primers designed for RT-PCR and RT-qPCR to validate differentially expressed miRNAs.

Primer name Primer sequence (5′→3′) Use
miR-96 GTCGTATCCAGTGCAGGGTCCGAGGT RT-PCR
ATTCGCACTGGATACGACAAACGA
miR-345 GTCGTATCCAGTGCAGGGTCCGAGGT RT-PCR
ATTCGCACTGGATACGACGCACTG
miR-127 GTCGTATCCAGTGCAGGGTCCGAGGT RT-PCR
ATTCGCACTGGATACGACAGCCAA
miR-31 GTCGTATCCAGTGCAGGGTCCGAGGT RT-PCR
ATTCGCACTGGATACGACCAGCTA
miR-96 TTTGGCACTAGCACATTCCG RT-qPCR
miR-345 GCTGACTCCTAGTCGCGC RT-qPCR
miR-127 TCGGATCCGTCTGAGCC RT-qPCR
miR-31 AGGCAAGATGCTGGCACC RT-qPCR
universal AP CTCAACTGGTGTCGTGGAGTC RT-qPCR
U6 F: GCTTCGGCAGCACATATACTAAAAT
R: CGCTTCACGAATTTGCGTGTCAT