Table 2. Primers designed for RT-PCR and RT-qPCR to validate differentially expressed miRNAs.
Primer name | Primer sequence (5′→3′) | Use |
---|---|---|
miR-96 | GTCGTATCCAGTGCAGGGTCCGAGGT | RT-PCR |
ATTCGCACTGGATACGACAAACGA | ||
miR-345 | GTCGTATCCAGTGCAGGGTCCGAGGT | RT-PCR |
ATTCGCACTGGATACGACGCACTG | ||
miR-127 | GTCGTATCCAGTGCAGGGTCCGAGGT | RT-PCR |
ATTCGCACTGGATACGACAGCCAA | ||
miR-31 | GTCGTATCCAGTGCAGGGTCCGAGGT | RT-PCR |
ATTCGCACTGGATACGACCAGCTA | ||
miR-96 | TTTGGCACTAGCACATTCCG | RT-qPCR |
miR-345 | GCTGACTCCTAGTCGCGC | RT-qPCR |
miR-127 | TCGGATCCGTCTGAGCC | RT-qPCR |
miR-31 | AGGCAAGATGCTGGCACC | RT-qPCR |
universal AP | CTCAACTGGTGTCGTGGAGTC | RT-qPCR |
U6 | F: GCTTCGGCAGCACATATACTAAAAT | |
R: CGCTTCACGAATTTGCGTGTCAT |