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. 2017 Nov 11;8(70):114924–114934. doi: 10.18632/oncotarget.22414

Figure 1. Viability and proliferation of lymphoma cells with 9-ING-41 treatment.

Figure 1

(A) Schematic representing GSK-3 up-stream regulators and down-stream targets and their role in cellular processes in cells. It should be noted that several of the target proteins are misregulated in cancer cells. Only target proteins relevant for this paper are shown for simplicity. (B-F) 10,000 cells (SUDHL-4, KPUM-UH1, Karpas 422, TMD8) per 96-well plate well were left untreated or treated with 1μM 9-ING-41 in triplicate, and the number of cells on days 1, 3, 5 and 7 were calculated using the MTS assay. Briefly, 20μL of MTS reagent was added to cells and incubated for 2 hours and, the absorbance at 490 nm (A490) was read using a Biotek plate reader. Error bars represent std. deviation between replicates. Day 3 viability is shown in B.