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. 2017 Dec 7;8(70):115054–115067. doi: 10.18632/oncotarget.23014

Figure 2. AR-mediated repression of YAP1 is associated with DNA methylation of the YAP1 promoter region.

Figure 2

(A) Immunofluorescence staining (IF) of YAP1 (red) and CK5 (green) in BHP, HNPC and CRPC tissue from human prostate cancer patients. Cell nuclei were visualized by DAPI staining. Representative stainings of YAP1 (red) and CK5 (green) in BHP, HNPC and CRPC tissue from human prostate cancer patients are shown. (B) Quantitation of YAP1 and CK5 expression from (A). (C) The schematic map of the YAP1 promoter-associated CpG island region. (D) Heatmaps showing the levels of YAP1 promoter methylation in four cell lines, HNPC cells (luminal and basal) and CRPC cells (luminal and basal). (E, F) DNA methylation PCR (MSP) show levels of YAP1 promotor methylation after treatment with DHT (10 nM), MDV3100 (100 nM) or 5AZA (5 μM). (G) Western blot analysis of YAP1 expression in LNCaP cell transfected with siRNA targeting DNMT3a (siDNMT3A). (H) qPCR measurement of YAP1 mRNA expression in siDNMT3a transfected LNCaP cells with or without DHT treatment. (I) YAP1 gene promoter driven luciferase reporter activity in LNCaP cell after treatment with DHT and upon transfection with siDNMT3a.