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. 2017 Dec 7;8(70):115054–115067. doi: 10.18632/oncotarget.23014

Figure 5. YAP1-mediated stem cell renewal depends on the TEAD-Interaction Domain of YAP1.

Figure 5

(A) Western blot analysis of SOX2 and Nanog protein expression in C4-2 cells transfected with siYAP1 and siControl. (B) qPCR measurements of mRNA expression levels of SOX2, Nanog and PSA in C4-2 cells transfected with control, YAP1wt or YAP1-S94A, YAP1-WW and YAP1-S369A mutant expression vectors as indicated. (C, D) SOX2 and Nanog gene promoter driven luciferase reporter activity in C4-2 cell transfected with expression vector for YPA1-WT or YAP1 mutants as indicated. (E) Sphere formation activity of C4-2 cells transfected with expression vectors for YAP1-WT and YAP1 variants as indicated. (F) Sphere formation activity and Western blot analysis of AR, YAP1 and SOX2 expression in C4-2 cells transfected with shAR and shYAP vectors. (G) Sphere formation activity of C4-2 cells transfected with YAP1wt vector and siRNA targeting SOX2.