Table 1. The expression levels of methylation in four cell lines, HNPC cell (luminal and basal), CRPC cell (luminal and basal).

name:YAP1		HNPC · basel	HNPC · luminal	CRPC · basel	CRPC · luminal
SampleID	CPG · Position	Y1	Y2	Y3	Y4
YAP1_CpG_1	102	0.47	0.55	0.22	0.3
YAP1_CpG_2	110	0.46	0.65	0.16	0.23
YAP1_CpG_3.4	139:141	0.45	0.57	0.25	0.27
YAP1_CpG_5	153	0.42	0.62	0.26	0.33
YAP1_CpG_6.7.8	161:166:170	0.47	0.41	0.27	0.21
YAP1_CpG_9	179	0.42	0.49	0.32	0.34
YAP1_CpG_10.11	187:189	0.47	0.56	0.19	0.28
YAP1_CpG_12	209	0.48	0.65	0.18	0.3
YAP1_CpG_15	260	0.51	0.55	0.21	0.29
YAP1_CpG_16.17	271:280	0.49	0.52	0.19	0.33
YAP1_CpG_18.19	286:290	0.47	0.62	0.17	0.27
YAP1_CpG_20	304	0.48	0.58	0.18	0.26
YAP1_CpG_21	308	0.46	0.56	0.16	0.32
YAP1_CpG_22	313	0.51	0.62	0.19	0.35
YAP1_CpG_23.24.25.26	338:342:344:351	0.45	0.61	0.25	0.29
YAP1_CpG_27	367	0.53	0.64	0.23	0.32
YAP1_CpG_28	373	0.49	0.63	0.24	0.28
YAP1_CpG_29.30.31.32	387:393:398:340	0.5	0.48	0.2	0.29
YAP1_CpG_33	412	0.58	0.55	0.22	0.27
YAP1_CpG_37.38.39	444:446:450	0.59	0.55	0.23	0.36
YAP1_CpG_40	457	0.59	0.55	0.24	0.32
YAP1_CpG_41	462	0.45	0.63	0.25	0.31
YAP1_CpG_42	472	0.48	0.6	0.26	0.33
YAP1_CpG_43	476	0.46	0.65	0.27	0.34
YAP1_CpG_44.45.46	483:487:491	0.48	0.64	0.18	0.35
YAP1_CpG_47.48.49.50	500:503:505:510	0.52	0.49	0.22	0.28

We design primer from -697 to -160 of YAP1 promtor enriched CG, and use a MassARRAY Compact MALDI-TOF(Sequenom), and spectra’s methylation ratioswe regenerated by the Epi-TYPER software v1.0 (Sequenom) in in four cell lines, HNPC cell (luminal and basal), CRPC cell (luminal and basal). Heatmaps showing higher methylation levels in luminal cells than those in basal cells.