Figure 1. Establishment of BEP2D cell model of lung cancer induced by cigarette smoke condensates.
(A) Giemsa staining for control and BEP2D cells induced with CSCs at 70th passage (P70, 200×). P70 cells show increase of nucleus to cytoplasm ratio and pathological mitosis. (B) MTT analysis for control and BEP2D cells induced by CSC at different generations. (C) Colony formation for control and BEP2D cells induced by CSC at different generations. (D) Apoptosis rate for control and BEP2D cells induced by CSC at different generations. Data are plotted as mean ± SD at least three independent experiments, *P<0.05, compared to control group.