Figure 3. Expression of Notch pathway members in BEP2D cells induced by CSC at different passage numbers.

(A) Western blots for protein expression of NOTCH signlaing pathway members, including NOTCH1, JAG1, Hey1, Hey2, Numb, JAG2, NOTCH2, Hes1, DNM1, RBPJ, in BEP2D cells from passage 0 to passage 70. GAPDH was used as a loading control. (B) qPCR analysis for comparisons of NOTCH pathway gene expression in BEP2D cells at passage 70 versus passage 0.