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. 2017 Dec 15;8(70):115384–115397. doi: 10.18632/oncotarget.23266

Figure 5. Enhanced Th17 cells in allografted liver tissues.

Figure 5

(A) IHC for IL-17 expression in liver tissues of recipients in the allograft and isograft groups at 7 days postoperatively. Positive cells were indicated by red arrows. (B) Statistical analysis of liver portal areas IL-17+ cell counts in IHC staining tissues. Plasma IL-17 concentrations (C) and mRNA levels of IL-17, IL-6 and IL-23 in liver tissues (D) of recipients (n=5) in the isograft or allograft group at the indicated time points were determined by ELISA or quantitative PCR. (E) The representative FCM results for the frequencies of Th17 cells (CD3+CD4+ IL-17+) in liver lymphocytes of the indicated groups at the 7 days after liver transplantation (n=5). (F) The statistical analysis for Th17 frequency in various groups at the indicated time points. (G) CXCL10, CCL20 and CXCR3 mRNA levels in liver tissues of recipients (n=5) in the indicated groups at days 1 and 7 after liver transplantation. *p<0.05.